DNA

Part:BBa_K2184020

Designed by: Margalit Mualem   Group: iGEM16_Danci-K8   (2016-09-25)

SNP for non-taster PTC taste

Engineered 15 SNPs (Single Nucleotide Polymorphism) in favor of this reaction. Each of the SNP’s that were selected enables identification of a certain taste out of the five known senses of taste, Each of the SNP’s that were selected Refer to people that unables identification of a certain taste out of the five known senses of taste. Using SNP segments – the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.

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usage and biology

engineered selected SNP (Single Nucleotide Polymorphism) in favor of this reaction. the SNP that were created enable identification of a certain taste out of the five known senses of taste, This selected SNP contein to PTC taste- rs713598


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pic 2- the population genetic polymorphism in the country in the context of this SNP.

Functional Parameters

Using SNP segments – the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. sample DNA is tested for the presence of a specific allele rs713598.

SNPs are screened using the Fluidigm BioMark apparatus (Fluidigm Corp.) with 16 Assay-specific TaqMan we will receive a fluorescence signal due to the release of a PROBE and or not. 67 DNA samples were tested using this method. Each sample was tested to check for the presence of alleles to identify different tastes in accordance to the sequence of the SNP ordered

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